TR-FRET assay. TR-FRET assay principle. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. Proceed with the following steps to optimize the instrument: 1. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Italic and boldface type are used for emphasis. EarlyTox Live Cell Assay (6. • Tel. Wavelength optimization. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. 1. M5. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. Fluorescence Microplate Reader Comparison Chart. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Issue: The audit trail in a SoftMax Pro GxP Software data file records a user as logging out of the system when the Auto Log Off prompt. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Published Dec 16, 2020 | Updated Dec 16, 2020. Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. +1 800-635-5577. . Turn on the microplate reader. 00 USD. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 3. No filter change with dual monochromator optics. . Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. 3: The cuvette chamber. The following document is intended to demonstrate setup of this instrument. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. $2,264. Add to Cart. Figure 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Category 2 and 3 microplate readers use gradient heating as well. We would like to show you a description here but the site won’t allow us. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. . SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataSpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Lower limits of detection for ATP determined using the ATPlite . book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax® M5 Multi-Mode Microplate Reader is capable of measuring UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, and TRF. Weight. It is available to all researchers. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. There were 12 replicate wells per dilution in both 96-well and 384- well plates. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Setup recommendations for SpectraMax® M5e. . 00 USD. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e• SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. 314 low lamp level warning. 0 mL). DOCUMENTATION. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The chamber. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Also refer to this Multimode Reader Comparison Chart. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Add to Cart. 2. Softmax Pro GxP Software 6. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Table of Contents. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. All reagents were brought to room temperature before use. . SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). . When the correct COM port is selected, the. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. HTRF is a proprietary time-resolved fluorescence technology that overcomes The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SpectraMax Glo and DuoLuc. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. Table 1. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Cytokines in medium released. The SpectraMax M5 Microplate Reader uses SoftMax® Pro. Figure 4: Transcreener ADP. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 2. view more . Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5 Multi-Mode Microplate Reader. edu) to be added as a trained user in iLab. Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. Detection modalities are shown in Table 1-1. The KeySpan USA-19HS is often required with new Windows 7. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. 3. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . 7" color, capacitive touch screen. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 3: The cuvette chamber. . Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Description 1. +44-118-944-8000. 314 low lamp level warning. SpectraMax M5 device. Multimode Reader Comparison Chart. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. The plate reader can measure multiple readouts, including UV. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Table of Contents. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity, permittingwidespreaduseincludingELISAs,proteinquantitation,endotoxinassays,and cytotoxicityassays. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. . The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 01) + 0. The cells were then washed and staining quantitation was performed using a fluorescent plate reader (SpectraMax M5 Molecular Devices, Sunnyvale, CA) at 492/520 excitation emission wavelengths. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. I have been using the SpectraMax M5 since 2011. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. With its 5-color monochromator detection head, MOLECULAR DEVICES SpectraMax M5 is the ideal choice for high-throughput applications, providing superior results in the analysis of a. SpectraMax® M5/M5e/FlexStation® 3 Instrument Settings Instrument Wavelength Settings EXC Wavelength 575 nm EMS Wavelength 620 nm Auto Cutoff 610 nm Optimized SpectraMax® M5/M5e Settings Readings 10-50 PMT Auto Auto Calibrate On Transcreener® HTS is a universal, high throughput biochemical assayUser guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Maximum signal with lowest background. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. free tracer decreases, resulting in an. ATP standard curves read on SpectraMax microplate readers. Introduction. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Table of Contents. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 00 USD. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. com A. Fluorescence spectra and UV–vis spectra were recorded on a SpectraMax ® M5 multi-mode microplate reader (Molecular Devices). The following document is intended to demonstrate setup of this. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. no. . Features. 1. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. SpectraMax Injector Cartridge with SmartInject™ Technology. 6 lbs. e. ISM Equipment Classification (Group 1, Class A) This equipment is. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. . Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. Select a rugged, lightweight model designed to be used in the field. 1. SpectraMax ABS Plus SpectraTest ABS2 v1. ; The top 3 importers of Spectramax are India with 365. Bookings are free, but you will need to use iLab to book. 1. DNA provides the same sensitivity as a spectrophotometer. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. We would like to show you a description here but the site won’t allow us. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Also refer to this Multimode Reader Comparison Chart. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. The dual monochromator optics allow the widest range of applications to be utilized for bior. The M5 can read volumes down to 2uL with the uMax Low Volume. Pick the best speed, sensitivity, and price for your needs. Click SoftMax Pro Software User Guide. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. . 1. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence intensity, glow luminescence, fluorescence polarization and time-resolved fluorescence (TRF). I have been using the SpectraMax M5 since 2011. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Download PDF versions of the SpectraMax M5 User Guides from this page. Dual monochromators provide the ability for. Wavelength Range. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9THE SPECTRAMAX PLUS Two ways to correct pathlength The SPECTRAmax PLUS gives you two options when making pathlength-cor-rected measurements: 1) putting the aqueous solvent into a clean 1 cm reference cuvette in the instrument’s cuvette port and having the instrument base the calcu-lations on the A 1000 and A 900Molecular Devices社の製品: マイクロプレート リーダー用ソフト SoftMax® Proに関するすべての情報をご覧ください。価格、見積もり、お近くの販売店を知るにはメーカーまたは本社に直接お問い合わせくだ. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. UV/Vis Photometric Performance:. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 2. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The excitation and the emission wavelengths on. Flexible for 6-well to 384-well microplates. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. SoftMax Pro. Detection of pro-inflammatory factors. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. SpectraMax M5 microplate reader. 1. Choose from a three- (M3), four- (M4), orAll general chemicals and solvents were commercially available and used as received. Instructions on the proper use of the SpectraMax M5 Plate Reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 2. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 19. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Five preferred detection modes. 7°C and the bottom to 36. We would like to show you a description here but the site won’t allow us. 72 MB. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. 6. Published Dec 16, 2020 | Updated Dec 16, 2020. . The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. The following document is intended to demonstrate setup of this instrument. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. SpectraMax® Microplate Readers and SoftMax® Pro Software. For wavelengths in the UV range above 220 nm,. Softmax Pro GxP Software 6. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The SpectraMax M5 is a microplate reader that can be used for various applications,. View the Molecular Devices website. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Page: 1/6. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Our absorbance plate readers feature our PathCheck Sensor. Refer to “Installing Detection Cartridges” in. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Small, unbound molecules rotate rapidly in solution (top), while large,. . iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Table of Contents. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Description 1. Description 1. This application note describes how to. Optimal instrument settings are the same for all three readers. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. . The SoftMax® Touch Software is installed in. 5. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. 95. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. 72 MB. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. In the M2, M5 and Plus 384, the light. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Microplate Reader Shelf. Statistical analyses The statistical analyses were performed using Graph Prism version 7. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Description 1. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. . HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Step 2. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. These all-inclusive assay kits are ready to use in assay validation or screening. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 2. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. 2. The assay applies fluorescence polarization to quantify Fc-containing IgG. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader. The following document is intended to demonstrate setup of this. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. The REF button has no valid meaning for. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. HEALTH AND SAFETY CONSIDERAIONSThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. . Plate chamber design of the SpectraMax category 1 microplate readers. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. , ~488 nm), has the optical. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. D 1. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. free shipping. . 306 plate air cal fail,low light. 5. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. The following document is intended to demonstrate setup of this instrument and provide representativeUsed together with the SpectraMax iD3 reader, the EarlyTox Cell Viability Assay Kits enable direct measurement of live and dead or apoptotic cells with a simple workflow and the increased throughput offered by a microplate format. In the M2, M5 and Plus. Wavelength optimization. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Bookings are. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Wavelength Range. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. In this product video, see how the SpectraMax M5 quickly reads samples. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. Step 4: Incubate at room temperature for 1 hour, protected from light. Table of Contents. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. . High tolerance for contaminants. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 26 5039228D Note. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the emission at 460 nm over 1 h. Cavicide (Warehouse, Cat # 79300360) 6. Multimode Readers Comparison Chart. The SpectraMax M5 Multi-Mode. Molecular Devices SpectraMax M5 Multimode Microplate Reader. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 2. Agilent VWorks Automation Control soft-ware manages all processes and incubation2 Luminescence integration time For supported SpectraMax M5 microplate readers (minimum firmware requirements apply), integration times of 3, 5, 7, and 10 seconds were added for luminescence read mode. Description 1. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. With optimized reagents, option to operate the readers. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. . The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. SpectraMax M2 and SpectraMax M2e are controlled by an external computer running SoftMax Pro software which provides integrated instrument control, data display, andQualify the fluorescence performance of the SpectraMax . .